The novel compositions of plant flavonoids and silver and gold nanoparticles substances

Work number - M 45 AWARDED

Submitted by Gromashevsky Institute of Epidemiology and Infectious Diseases, the National Academy of Medical Sciences of Ukraine

Authors: Starosyla DB, PhD, Rybachuk AV, PhD

The authors studied the properties and mechanisms of antiviral activity of the novel group of antiviral substances – compositions of plant flavonoids. The active structure of these plant flavonoids was determined and the antiviral effects of the natural flavonoids and their synthetic analogues were studied in the in vivo and in vitro models of the experimental viral infections such as influenza virus, herpes virus type 1 and 2, papillomavirus, hepatitis virus C. The efficacy of silver and gold nanoparticles was assessed for the treatment and prevention of pyo-inflammatory complications of the traumatic injuries of the lower jaw.

The mechanisms of antiviral activity of the biologically active substances were elucidated. In particular, flavonoids under study inhibit neuraminidase of influenza virus, inhibit RNA and DNA synthesis. These substances are active interferon inducers with the decreased expression of 2-5-oligoadenylate synthetase gene.

For the first time was provided the system laboratory-experimental of studies for silver and gold nanoparticles experimental substancestopical application ofmandible traumatic fractures purulent-inflammatorycomplicationstreatment and prevention.

In in vitro, in vivo and ex vivo studies, the high antibacterial, anti-inflammatory and reparative activities of silver and gold nanoparticles, 30 nm in size, used as single agents or in combination have been proved exceeding the efficacy of the commercial antiseptics. The mechanisms of antibacterial, anti-inflammatory and reparative activities of silver and gold nanoparticles have been disclosed. It was demonstrated that bacterial cells from clinical isolates are capable of silver and gold nanoparticles active accumulation, such accumulation being the component of the mechanism of antibacterial activity of the nanoparticles under study.

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