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Молекулярные механизмы корректирующего действия никотинамида и холекальциферола при метаболических нарушениях, индуцируемых сахарным диабетом

Work number - M 77

Authors: M. Guzik, Labudzynskyy DO, TN Tykhonenko

Presented by the Institute of Biochemistry. OV Palladin NAS of Ukraine

Currently, prevention and treatment of diabetes mellitus (DM) is an actual medical and social problem, because diabetes is referred to as a disease that often causes early disability and mortality among people from all over the world. However, the mechanisms underlying the development of diabetes and its complications are not fully understood and new treatment approaches are needed.

Studying the contribution of poly-ADP-ribosylation of nuclear proteins to cell dysfunction in type 1 diabetes remains one of the crucial problems of modern biochemistry. For the first time it was systematically studied the level of protein poly-ADP-ribosylation in tissues of different organs in relation to other metabolic processes and experimentally proved the applicability of nicotinamide and cholecalciferol treatments to correct diabetes-induced disorders. In addition, the comprehensive therapeutic effect of cholecalciferol in animals with diabetes was investigated on the background of pronounced vitamin D3 deficiency elicited by the disease. It was shown that diabetes-induced changes were accompanied by activation of NF-κB-associated signaling pathways and increased levels of regulatory proteins and inflammatory cytokines in serum and different tissues. Administration of cholecalciferol in therapeutic doses in diabetes led to normalization of metabolic processes in the liver and bone tissue. These effects were realized through genomic mechanisms in regulation of cellular functions, including poly-ADP-ribosylation of proteins as well as via influencing the functions of immune system (immunomodulatory action).
Studying the effects of nicotinamide and cholecalciferol treatment on experimental diabetes made it possible to assert that these compounds possess anti-inflammatory, antioxidant, immunomodulatory and cytoprotective properties. The study can serve as a basis for developing a comprehensive drug therapy for supporting treatment of diabetes and its complications that can improve the quality of life of patients with diabetes.

The research results presented in 43 publications, including 16 articles in refereed journals (7 of them in journals cited in Scopus). The total citation index is 2 under the database Scopus. The works of authors cited in 5 scientific journals.