Work number - M 48 FILED
Authors:Krasylenko Y.A. , Horiunova I.I., Plohovskaya S.H.
Presented by State Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine
The paper presents the results of studies of the effect of modulators content synthase inhibitor, a marker of nitric oxide activity, metals polluting soil – cadmium, nickel, copper and zinc, as well as low temperature + 40C and + 0,50Сgrowth, in vivo morphology and cytoskeleton organization components plant cell, the example of the cells of the roots of seedlings Arabidopsis thaliana. Revealed that microtubules plant cells are sensitive to changes in intracellular nitric oxide. Describe the changes of growth and morphology of A. thaliana root key and organization of microtubules in cells due to irradiation of UV-B. Demonstrated functional relationship between nitrogen oxides and microtubule stability to UV-B.
For the first time shown that the cytoskeleton of cells of different growth zones roots are sensitive to the effects of toxic metal ions. The immediate impact on the metal components of the cytoskeleton is one reason dozo- and chaso- dependent disorders morphology of the main root. For the first time to study the characteristics lifetime of actin filaments in cells of A. thaliana after exposure to temperatures 4◦С+ and + 0,5◦С. Shown the negative impact of the combined effect of low temperature oxide and interceptor for the organization of microfilaments. Established that actin filaments disorientation increases in their combined action.
The results can be used to further study the molecular and cellular mechanisms of plant resistance involving nitric oxide and to search for new biologically active compounds that affect the plant components of the cytoskeleton. Lay the foundation for studying the possibility of using nitric oxide donor in biotechnology with the aim of regulating plant growth and enhance their resistance to various stressors, including UV-B and cold. Studies of exposure to toxic metals and cold on the growth and morphology of the main roots of plants, as well as the organization of the components of the cytoskeleton, will make it possible to develop new, more effective strategies to monitor the content of metals in soils and to combat the common abiotic factors that cause unwashed damage to cultural plants, such as temperature and pollution-metal pollutants.