Work number - M 13 FILED
Presented Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine
The state of micro- and ultrastructure, antigenic properties, biomechanical characteristics of porcine pericardial tissue (Pr) and aortic valve leaflets (AVL) under the influence of low temperatures, radiation by electron flow and their combined effect were assessed in a complex. The expression rate of markers of collagens, elastin, endothelial cells, α-Gal-positive cells in tissues was determined. In vivo tissue reaction was studied in response to implantation of tissues (subcutaneous xenoimplantation, n = 85), the features of biointegration, functional efficiency of xenopericardium after combined effect (plastic of rabbit wall urinary bladder defect, n = 19) were investigated.
After the action of low temperatures, there were disorganization processes of Pr and AVL collagen and elastin fibers. Fibers and cellular elements retained the antigenic structure with pronounced expression of markers of collagen I, III, elastin and CD31. After radiation, severe damage to the cells were developed, namely: necrobiosis and necrosis, total desquamation of endothelial cells. Combined action resulted in a less pronounced diffuse expression of markers, providing a high degree of tissue devitalization.
Extracellular matrix of tissues devitalized by combined effect, preserved its integrity and possessed physical and mechanical properties characteristic of native tissues in the longitudinal and transverse directions. In this case, the devitalized tissues demonstrated the strengthening of elastic characteristics, retained strength, the ability to deform in all the directions, resistance to breakage and twisting.
After a combined effect, the Pr tissue exhibits inert properties after implantation: no signs of rejection, maintenance of operating loads in conditions of aggressive environment, performance of the carcass function in the resected area and ensuring reliable restoration of the anatomical integrity and functional parameters of the urinary bladder.
Number of publications: 22 works, including 5 scientific articles (including 4 articles in international scientific publications and 4 articles in journals indexed in Scopus and/or Web of Science), 17 abstracts of reports. Total index of citations of author/ h-index of the work: Manchenko A.O. - h-index (Scopus) - 6/2, h-index (Google Scholar) - 12/2.